Mastering Gel Filtration Chromatography: A Comprehensive Guide with 30 MCQs and Solutions
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What is the principle behind Gel Filtration Chromatography?
- a) Separation based on charge
- b) Separation based on size
- c) Separation based on hydrophobicity
- d) Separation based on affinityqqq
Solution: b) Separation based on size Explanation: Gel Filtration Chromatography separates molecules based on their size, with larger molecules eluting first because they are not able to enter the pores of the gel matrix.
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Which of the following terms is commonly used interchangeably with Gel Filtration Chromatography?
- a) Ion Exchange Chromatography
- b) Size Exclusion Chromatography
- c) Affinity Chromatography
- d) Reverse Phase Chromatography
Solution: b) Size Exclusion Chromatography Explanation: Gel Filtration Chromatography is often referred to as Size Exclusion Chromatography because it separates molecules based on their size.
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In Gel Filtration Chromatography, what is the stationary phase?
- a) The sample mixture
- b) The gel matrix
- c) The eluent
- d) The mobile phase.
Solution: b) The gel matrix Explanation: The gel matrix serves as the stationary phase in Gel Filtration Chromatography, where molecules are separated based on their size as they pass through the pores of the gel.
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Which of the following statements regarding Gel Filtration Chromatography is true?
- a) Smaller molecules elute first.
- b) It is based on specific binding interactions.
- c) It requires a specific ligand.
- d) It separates molecules based on charge.
Solution: a) Smaller molecules elute first. Explanation: Gel Filtration Chromatography separates molecules based on their size, with smaller molecules taking longer to pass through the gel matrix and eluting later.
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What happens to large molecules in Gel Filtration Chromatography?
- a) They elute first.
- b) They are retained within the gel matrix.
- c) They interact with the stationary phase.
- d) They do not enter the column.
Solution: b) They are retained within the gel matrix.
Explanation: Large molecules cannot enter the pores of the gel matrix and therefore become trapped, eluting later than smaller molecules. -
Which of the following factors affects the resolution in Gel Filtration Chromatography?
- a) pH of the mobile phase
- b) Temperature
- c) Size of the gel beads
- d) Type of eluent
Solution: c) Size of the gel beads Explanation: The size of the gel beads directly impacts the resolution of Gel Filtration Chromatography, with smaller beads providing higher resolution.
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What type of molecules is best separated using Gel Filtration Chromatography?
- a) Molecules with similar sizes
- b) Charged molecules
- c) Hydrophobic molecules
- d) Large molecules.qqq
Solution: a) Molecules with similar sizes Explanation: Gel Filtration Chromatography is ideal for separating molecules with similar sizes but different molecular weights.
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What is the function of the gel matrix in Gel Filtration Chromatography?
- a) To bind to specific molecules
- b) To create an electric field
- c) To separate molecules based on charge
- d) To provide a porous network for separation
Solution: d) To provide a porous network for separation Explanation: The gel matrix acts as a porous network through which molecules can pass, with larger molecules being excluded from the pores and eluting first.
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Which of the following is a common gel material used in Gel Filtration Chromatography?
- a) Sepharose
- b) Silica
- c) Polystyrene
- d) Agarose
Solution: a) Sepharose Explanation: Sepharose is a common gel material used in Gel Filtration Chromatography due to its high chemical stability and uniform pore size distribution.
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How does the elution volume change with increasing molecular weight in Gel Filtration Chromatography?
- a) Increases
- b) Decreases
- c) Remains constant
- d) Fluctuates
Solution: a) Increases Explanation: Larger molecules have greater difficulty entering the pores of the gel matrix and thus elute later, resulting in an increase in elution volume with increasing molecular weight.
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What is the primary disadvantage of Gel Filtration Chromatography?
- a) Low resolution
- b) Long run times
- c) Limited sample capacity
- d) Limited separation range.
Solution: c) Limited sample capacity Explanation: Gel Filtration Chromatography has a limited sample capacity due to the finite volume of the gel matrix and the need for molecules to enter the pores for separation.
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Which of the following elution buffers is commonly used in Gel Filtration Chromatography?
- a) High salt concentration
- b) Low pH
- c) High pH
- d) Buffer with low ionic strength
Solution: d) Buffer with low ionic strength Explanation: Elution buffers with low ionic strength are commonly used in Gel Filtration Chromatography to minimize non-specific interactions and promote efficient elution of separated molecules.
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What is the role of the sample size in Gel Filtration Chromatography?
- a) It affects the elution volume
- b) It affects the resolution
- c) It affects the run time
- d) It affects the pH of the eluent
Solution: a) It affects the elution volume Explanation: The size of the sample can affect the elution volume in Gel Filtration Chromatography, with larger sample sizes potentially leading to broader peaks and increased run times.
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What is the primary determinant of a molecule's elution time in Gel Filtration Chromatography?
- a) Its molecular weight
- b) Its charge
- c) Its hydrophobicity
- d) Its shape
Solution: a) Its molecular weight Explanation: In Gel Filtration Chromatography, a molecule's elution time is primarily determined by its molecular weight, with larger molecules eluting earlier than smaller ones.
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Which of the following statements regarding Gel Filtration Chromatography is false?
- a) It separates molecules based on size
- b) It requires a specific binding interaction
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